PMID: 32264863

Comprehensive routine diagnostic screening to identify predictive mutations, gene amplifications, and microsatellite instability in FFPE tumor material.

BMC cancer

Steeghs, Elisabeth M P EMP, Kroeze, Leonie I LI, Tops, Bastiaan B J BBJ, van Kempen, Leon C LC, Ter Elst, Arja A, Kastner-van Raaij, Annemiek W M AWM, Hendriks-Cornelissen, Sandra J B SJB, Hermsen, Mandy J W MJW, Jansen, Erik A M EAM, Nederlof, Petra M PM, Schuuring, Ed E, Ligtenberg, Marjolijn J L MJL, Eijkelenboom, Astrid A

2020-04-07

259 variants were identified in this publication

Variant ID	Gene	cDNA	Protein	Consequence	Exon	Intron
17-7577524-T-C	TP53	c.757A>G	p.T253A	Missense	7/11	-
7-55242484-ATCT-A	EGFR	c.2254_2256delTCT	p.S752del	InFrameDeletion	19/28	-
17-7577577-T-C	TP53	c.704A>G	p.N235S	Missense	7/11	-
17-7578527-A-G	TP53	c.403T>C	p.C135R	Missense	5/11	-
17-7578515-T-C	TP53	c.415A>G	p.K139E	Missense	5/11	-
1-243859016-C-T	AKT3	c.49G>A	p.E17K	Missense	3/14	-
17-7577079-C-A	TP53	c.859G>T	p.E287*	Nonsense	8/11	-
7-140453139-G-A	BRAF	c.1796C>T	p.T599I	Missense	15/18	-
3-12645693-G-A	RAF1	c.776C>T	p.S259F	Missense	7/17	-
7-55242487-C-T	EGFR	c.2257C>T	p.P753S	Missense	19/28	-
17-7578274-T-C	TP53	c.575A>G	p.Q192R	Missense	6/11	-
7-55242469-TTAAGAGAAG-C	EGFR	c.2239_2248delinsC	p.L747_A750delinsP	MultiAAMissense	19/28	-
10-89711916-T-A	PTEN	c.534T>A	p.Y178*	Nonsense	6/9	-
17-7578460-A-T	TP53	c.470T>A	p.V157D	Missense	5/11	-
10-89717642-A-T	PTEN	c.667A>T	p.K223*	Nonsense	7/9	-
17-7578518-C-G	TP53	c.412G>C	p.A138P	Missense	5/11	-
17-7577545-T-G	TP53	c.736A>C	p.M246L	Missense	7/11	-
4-55593613-T-A	KIT	c.1679T>A	p.V560D	Missense	11/21	-
17-7578265-A-T	TP53	c.584T>A	p.I195N	Missense	6/11	-
17-7573982-C-A	TP53	c.1045G>T	p.E349*	Nonsense	10/11	-
17-7579395-G-A	TP53	c.292C>T	p.P98S	Missense	4/11	-
4-55593608-GGTT-G	KIT	c.1679_1681delTTG	p.V560del	InFrameDeletion	11/21	-
4-55593604-GGAA-G	KIT	c.1672_1674delAAG	p.K558del	InFrameDeletion	11/21	-
10-89720852-C-T	PTEN	c.1003C>T	p.R335*	Nonsense	8/9	-
12-25398266-G-T	KRAS	c.53C>A	p.A18D	Missense	2/5	-
17-7574012-C-A	TP53	c.1015G>T	p.E339*	Nonsense	10/11	-
17-7578504-CAGG-C	TP53	c.424_426delCCT	p.P142del	InFrameDeletion	5/11	-
17-7577118-C-G	TP53	c.820G>C	p.V274L	Missense	8/11	-
17-7574012-C-T	TP53	c.1015G>A	p.E339K	Missense	10/11	-
17-7578474-C-CG	TP53	c.455_455dup	p.P153Afs*28	Frameshift	5/11	-
17-7578463-CGGGTGCCGGGCGG-C	TP53	c.454_466del13	p.P152Afs*14	Frameshift	5/11	-
7-116411998-A-G	MET	c.2983A>G	p.M995V	Missense	14/21	-
17-7577535-C-T	TP53	c.746G>A	p.R249K	Missense	7/11	-
17-7577498-C-T	TP53	c.782+1G>A	-	IntronicSNV	-	7/10
17-7577548-C-G	TP53	c.733G>C	p.G245R	Missense	7/11	-
7-140453132-ATTT-A	BRAF	c.1801_1803delAAA	p.K601del	InFrameDeletion	15/18	-
17-7578236-A-G	TP53	c.613T>C	p.Y205H	Missense	6/11	-
17-7579373-C-A	TP53	c.314G>T	p.G105V	Missense	4/11	-
7-116340270-G-A	MET	c.1132G>A	p.V378I	Missense	2/21	-
4-55593659-ACTT-A	KIT	c.1727_1729delTTC	p.L576del	InFrameDeletion	11/21	-
17-7579329-T-C	TP53	c.358A>G	p.K120E	Missense	4/11	-
17-7577514-GTGA-G	TP53	c.764_766delTCA	p.I255del	InFrameDeletion	7/11	-
17-7577580-T-G	TP53	c.701A>C	p.Y234S	Missense	7/11	-
3-178921548-G-A	PIK3CA	c.1030G>A	p.V344M	Missense	5/21	-
17-7577568-C-G	TP53	c.713G>C	p.C238S	Missense	7/11	-
17-7578371-C-T	TP53	c.559G>A	p.G187S	Missense	5/11	-
17-7578542-G-A	TP53	c.388C>T	p.L130F	Missense	5/11	-
17-7578457-C-G	TP53	c.473G>C	p.R158P	Missense	5/11	-
3-178936095-A-T	PIK3CA	c.1637A>T	p.Q546L	Missense	10/21	-
3-178936096-G-C	PIK3CA	c.1638G>C	p.Q546H	Missense	10/21	-
7-55242469-ATTAAGAGAAGCAACATCT-A	EGFR	c.2239_2256del18	p.L747_S752del	InFrameDeletion	19/28	-
15-66729093-CTGGAGA-C	MAP2K1	c.303_308delGGAGAT	p.E102_I103del	InFrameDeletion	3/11	-
17-7578177-C-T	TP53	c.672G>A	p.E224=	Synonymous	6/11	-
17-7579377-G-A	TP53	c.310C>T	p.Q104*	Nonsense	4/11	-
17-7577093-C-G	TP53	c.845G>C	p.R282P	Missense	8/11	-
4-55144148-C-G	PDGFRA	c.1977C>G	p.N659K	Missense	14/23	-
1-11184573-G-A	MTOR	c.6644C>T	p.S2215F	Missense	47/58	-
3-178921552-A-T	PIK3CA	c.1034A>T	p.N345I	Missense	5/21	-
10-89692905-G-C	PTEN	c.389G>C	p.R130P	Missense	5/9	-
11-534285-C-A	HRAS	c.38G>T	p.G13V	Missense	2/6	-
17-7577508-T-G	TP53	c.773A>C	p.E258A	Missense	7/11	-
3-178952138-C-T	PIK3CA	c.3193C>T	p.H1065Y	Missense	21/21	-
17-7579360-G-GA	TP53	c.326_326dup	p.R110Pfs*39	Frameshift	4/11	-
17-7578176-C-A	TP53	c.672+1G>T	-	IntronicSNV	-	6/10
17-7576852-C-A	TP53	c.993+1G>T	-	IntronicSNV	-	9/10
15-90631917-T-TC	IDH2	c.435_435dupG	p.T146Dfs*126	Frameshift	4/11	-
17-7578370-C-A	TP53	c.559+1G>T	-	IntronicSNV	-	5/10
17-7574002-C-G	TP53	c.1025G>C	p.R342P	Missense	10/11	-
17-7578224-T-A	TP53	c.625A>T	p.R209*	Nonsense	6/11	-
17-7578467-T-G	TP53	c.463A>C	p.T155P	Missense	5/11	-
10-89692919-A-G	PTEN	c.403A>G	p.I135V	Missense	5/9	-
17-7577544-A-G	TP53	c.737T>C	p.M246T	Missense	7/11	-
15-66727443-T-G	MAP2K1	c.159T>G	p.F53L	Missense	2/11	-
17-7578458-G-C	TP53	c.472C>G	p.R158G	Missense	5/11	-
17-7578449-C-A	TP53	c.481G>T	p.A161S	Missense	5/11	-
17-7577106-G-T	TP53	c.832C>A	p.P278T	Missense	8/11	-
3-178952018-A-G	PIK3CA	c.3073A>G	p.T1025A	Missense	21/21	-
17-7579494-T-A	TP53	c.193A>T	p.R65*	Nonsense	4/11	-
17-7578440-T-C	TP53	c.490A>G	p.K164E	Missense	5/11	-
17-7578402-G-C	TP53	c.528C>G	p.C176W	Missense	5/11	-
17-7577569-A-G	TP53	c.712T>C	p.C238R	Missense	7/11	-
17-7577142-C-A	TP53	c.796G>T	p.G266*	Nonsense	8/11	-
17-7577081-T-C	TP53	c.857A>G	p.E286G	Missense	8/11	-
12-25380282-G-T	KRAS	c.176C>A	p.A59E	Missense	3/5	-
17-7578263-G-C	TP53	c.586C>G	p.R196G	Missense	6/11	-
12-25398279-C-A	KRAS	c.40G>T	p.V14L	Missense	2/5	-
2-29443622-T-G	ALK	c.3595A>C	p.M1199L	Missense	23/29	-
7-140481408-G-A	BRAF	c.1400C>T	p.S467L	Missense	11/18	-
7-140477854-A-C	BRAF	c.1454T>G	p.L485W	Missense	12/18	-
4-55593600-CAGTGGA-C	KIT	c.1669_1674delTGGAAG	p.W557_K558del	InFrameDeletion	11/21	-
17-7577077-TCTC-T	TP53	c.859_861delGAG	p.E287del	InFrameDeletion	8/11	-
17-7578554-A-C	TP53	c.376T>G	p.Y126D	Missense	5/11	-
17-7574021-C-A	TP53	c.1006G>T	p.E336*	Nonsense	10/11	-
17-7579485-C-A	TP53	c.202G>T	p.E68*	Nonsense	4/11	-
4-55593606-GAAGGTTGTT-G	KIT	c.1672_1680delAAGGTTGTT	p.K558_V560del	InFrameDeletion	11/21	-
4-55593666-TATG-T	KIT	c.1735_1737delGAT	p.D579del	InFrameDeletion	11/21	-
17-37880997-G-GTGT	ERBB2	c.2326_2327insTGT	p.G776delinsVC	MultiAAMissense	20/27	-
10-89711875-G-T	PTEN	c.493G>T	p.G165*	Nonsense	6/9	-
10-89720808-T-C	PTEN	c.959T>C	p.L320S	Missense	8/9	-
17-7577566-T-TA	TP53	c.714dupT	p.N239*	Nonsense	7/11	-
10-89717699-G-T	PTEN	c.724G>T	p.E242*	Nonsense	7/9	-
17-7578530-A-C	TP53	c.400T>G	p.F134V	Missense	5/11	-
19-3118954-G-A	GNA11	c.638G>A	p.R213Q	Missense	5/7	-
3-12645688-G-A	RAF1	c.781C>T	p.P261S	Missense	7/17	-
17-7578541-AG-A	TP53	c.388delC	p.L130Sfs*40	Frameshift	5/11	-
10-89720798-GTACT-G	PTEN	c.955_958delACTT	p.T319*	Frameshift	8/9	-
17-7577100-TC-T	TP53	c.837delG	p.R280Efs*65	Frameshift	8/11	-
17-7579409-AG-A	TP53	c.277delC	p.L93Cfs*30	Frameshift	4/11	-
17-7578474-CG-C	TP53	c.455delC	p.P152Rfs*18	Frameshift	5/11	-
17-7577095-GTC-G	TP53	c.841_842delGA	p.D281Pfs*24	Frameshift	8/11	-
17-7578504-AG-A	TP53	c.425delC	p.P142Lfs*28	Frameshift	5/11	-
10-89720811-C-CA	PTEN	c.968dupA	p.N323Kfs*2	Frameshift	8/9	-
10-89717769-TA-T	PTEN	c.800delA	p.K267Rfs*9	Frameshift	7/9	-
17-7579314-T-C	TP53	c.373A>G	p.T125A	Missense	4/11	-
17-7579320-TCA-T	TP53	c.365_366delTG	p.V122Dfs*26	Frameshift	4/11	-
17-7577140-TC-T	TP53	c.797delG	p.G266Dfs*79	Frameshift	8/11	-
10-89720855-T-TA	PTEN	c.1007_1007dupA	p.Y336*	Nonsense	8/9	-
2-29445412-C-T	ALK	c.3421G>A	p.D1141N	Missense	21/29	-
4-55593604-TGGAAGG-T	KIT	c.1670_1675delGGAAGG	p.W557_V559delinsF	MultiAAMissense	11/21	-
17-7573984-A-G	TP53	c.1043T>C	p.L348S	Missense	10/11	-
17-37879583-C-G	ERBB2	c.1958C>G	p.S653C	Missense	17/27	-
10-89692924-T-G	PTEN	c.408T>G	p.C136W	Missense	5/9	-
12-25380279-CCTG-C	KRAS	c.176_178delCAG	p.A59del	InFrameDeletion	3/5	-
17-7577545-TGCC-T	TP53	c.733_735delGGC	p.G245del	InFrameDeletion	7/11	-
12-133250268-G-A	POLE	c.1252C>T	p.P418S	Missense	13/49	-
12-25378648-T-C	KRAS	c.350A>G	p.K117R	Missense	4/5	-
17-7578242-C-A	TP53	c.607G>T	p.V203L	Missense	6/11	-
2-29443669-A-G	ALK	c.3548T>C	p.I1183T	Missense	23/29	-
4-55594233-T-G	KIT	c.1936T>G	p.Y646D	Missense	13/21	-
17-7578241-A-T	TP53	c.608T>A	p.V203E	Missense	6/11	-
15-66729108-G-A	MAP2K1	c.316G>A	p.A106T	Missense	3/11	-
15-66727464-GGGA-G	MAP2K1	c.182_184delGAG	p.G61del	InFrameDeletion	2/11	-
1-11174416-G-A	MTOR	c.7259C>T	p.A2420V	Missense	53/58	-
17-7578210-TCG-CCA	TP53	c.637_639delCGAinsTGG	p.R213W	Missense	6/11	-
7-140453131-ATTTCAC-A	BRAF	c.1798_1803delGTGAAA	p.V600_K601del	InFrameDeletion	15/18	-
7-140481417-C-G	BRAF	c.1391G>C	p.G464A	Missense	11/18	-
10-89711945-A-G	PTEN	c.563A>G	p.Y188C	Missense	6/9	-
4-55597561-G-A	KIT	c.2209G>A	p.D737N	Missense	15/21	-
4-55594261-A-C	KIT	c.1964A>C	p.N655T	Missense	13/21	-
10-89692830-G-T	PTEN	c.314G>T	p.C105F	Missense	5/9	-
7-55249134-C-T	EGFR	c.2432C>T	p.S811F	Missense	20/28	-
19-3118943-G-T	GNA11	c.627G>T	p.Q209H	Missense	5/7	-
17-7573976-T-A	TP53	c.1051A>T	p.K351*	Nonsense	10/11	-
X-47426121-C-A	ARAF	c.641C>A	p.S214Y	Missense	7/16	-
17-7574029-CG-C	TP53	c.997delC	p.R333Vfs*12	Frameshift	10/11	-
10-89693002-CA-C	PTEN	c.491delA	p.K164Rfs*3	Frameshift	5/9	-
10-89692948-A-C	PTEN	c.432A>C	p.K144N	Missense	5/9	-
4-55593602-AGTGGAAGGTTGT-A	KIT	c.1668_1679del12	p.Q556_V560delinsH	MultiAAMissense	11/21	-
17-7577530-TG-T	TP53	c.750delC	p.I251Sfs*94	Frameshift	7/11	-
15-66729098-GATC-G	MAP2K1	c.308_310delTCA	p.I103del	InFrameDeletion	3/11	-
17-7576902-G-C	TP53	c.944C>G	p.S315C	Missense	9/11	-
7-55259532-G-C	EGFR	c.2590G>C	p.A864P	Missense	21/28	-
17-7578369-A-C	TP53	c.559+2T>G	-	IntronicSNV	-	5/10
17-7579419-A-AG	TP53	c.267_267dup	p.S90Lfs*59	Frameshift	4/11	-
17-7578259-A-T	TP53	c.590T>A	p.V197E	Missense	6/11	-
17-7578468-GC-G	TP53	c.461delG	p.G154Afs*16	Frameshift	5/11	-
17-7577549-GC-G	TP53	c.731delG	p.G244Afs*3	Frameshift	7/11	-
17-7577545-AT-A	TP53	c.736delA	p.M246*	Frameshift	7/11	-
7-55242419-T-G	EGFR	c.2189T>G	p.L730R	Missense	19/28	-
4-1803565-G-A	FGFR3	c.743G>A	p.R248H	Missense	7/18	-
17-7578490-AC-A	TP53	c.439delG	p.V147Lfs*23	Frameshift	5/11	-
17-37879655-G-A	ERBB2	c.2030G>A	p.R677Q	Missense	17/27	-
10-89692981-T-TG	PTEN	c.469_469dupG	p.E157Gfs*23	Frameshift	5/9	-
10-89712017-G-A	PTEN	c.634+1G>A	-	IntronicSNV	-	6/8
12-133252403-G-A	POLE	c.1024C>T	p.H342Y	Missense	11/49	-
10-89693002-C-CA	PTEN	c.491_491dupA	p.V166Sfs*14	SpliceDonorInsertion	5/9	-
10-89712015-C-CT	PTEN	c.633_634insT	p.N212*	SpliceDonorInsertion	6/9	-
17-7578274-TGA-T	TP53	c.573_574delTC	p.Q192Afs*16	Frameshift	6/11	-
17-37880993-GGCT-G	ERBB2	c.2324_2326delCTG	p.A775del	InFrameDeletion	20/27	-
12-25380256-T-A	KRAS	c.202A>T	p.R68W	Missense	3/5	-
12-133236074-C-A	POLE	c.3082G>T	p.E1028*	Nonsense	26/49	-
1-162745588-G-A	DDR2	c.2003G>A	p.R668H	Missense	15/18	-
17-7577103-CAGG-C	TP53	c.832_834delCCT	p.P278del	InFrameDeletion	8/11	-
17-7579505-AT-A	TP53	c.182delA	p.D61Vfs*62	Frameshift	4/11	-
17-7578451-A-C	TP53	c.479T>G	p.M160R	Missense	5/11	-
2-29436869-C-T	ALK	c.3724G>A	p.E1242K	Missense	24/29	-
19-40762911-C-T	AKT2	c.97G>A	p.G33S	Missense	3/14	-
17-7578375-CGCT-C	TP53	c.553_555delAGC	p.S185del	InFrameDeletion	5/11	-
10-89717611-TC-T	PTEN	c.638delC	p.P213Lfs*8	Frameshift	7/9	-
17-7577594-ACAGTCAGAGC-A	TP53	c.677_686delGCTCTGACTG	p.G226Vfs*18	Frameshift	7/11	-
17-7577607-A-G	TP53	c.674T>C	p.V225A	Missense	7/11	-
4-55593637-ATGTTTACATAGACCCAACACAACT-A	KIT	c.1705_1728del24	p.V569_L576del	InFrameDeletion	11/21	-
17-7579316-C-CA	TP53	c.370dupT	p.C124Lfs*25	Frameshift	4/11	-
17-7578229-TC-T	TP53	c.619delG	p.D207Mfs*40	Frameshift	6/11	-
15-66727450-C-A	MAP2K1	c.166C>A	p.Q56K	Missense	2/11	-
10-89717636-A-T	PTEN	c.661A>T	p.K221*	Nonsense	7/9	-
7-55242484-T-A	EGFR	c.2254T>A	p.S752T	Missense	19/28	-
17-7578505-AGGGCAGGTCTTGGCC-A	TP53	c.411_425del15	p.A138_P142del	InFrameDeletion	5/11	-
17-7579523-AG-A	TP53	c.164delC	p.T55Mfs*68	Frameshift	4/11	-
17-7576916-TG-T	TP53	c.930delC	p.N310Kfs*35	Frameshift	9/11	-
17-7577134-GTTCCGTCCCAGTAGA-G	TP53	c.789_803del15	p.L264_N268del	InFrameDeletion	8/11	-
17-7577132-CTGT-C	TP53	c.803_805delACA	p.N268del	InFrameDeletion	8/11	-
17-7577583-TG-T	TP53	c.697delC	p.H233Tfs*14	Frameshift	7/11	-
4-55594233-T-A	KIT	c.1936T>A	p.Y646N	Missense	13/21	-
7-140477866-G-C	BRAF	c.1442C>G	p.A481G	Missense	12/18	-
10-89692989-T-G	PTEN	c.473T>G	p.V158G	Missense	5/9	-
4-55593672-TCAC-T	KIT	c.1738_1740delCAC	p.H580del	InFrameDeletion	11/21	-
1-162748449-C-A	DDR2	c.2363C>A	p.P788H	Missense	17/18	-
4-55593653-CAACACAACTTCCTTATGATCA-C	KIT	c.1719_1739del21	p.T574_H580del	InFrameDeletion	11/21	-
11-533832-CC-TT	HRAS	c.223_224delinsAA	p.G75K	Missense	3/6	-
17-7577095-GT-CTCCCTTA	TP53	c.841_843delinsGTAAGGGAG	p.D281delinsVRE	MultiAAMissense	8/11	-
4-55594233-TAC-GAGGTACTTAGCCAT	KIT	c.1936_1938delinsGAGGTACTTAGCCAT	p.Y646delinsEVLSH	MultiAAMissense	13/21	-
4-55144077-A-G	PDGFRA	c.1906A>G	p.S636G	Missense	14/23	-
4-55599343-TGTGGTTAAA-T	KIT	c.2471_2479delTGGTTAAAG	p.V824_K826del	InFrameDeletion	17/21	-
4-55599350-TAAA-T	KIT	c.2476_2478delAAA	p.K826del	InFrameDeletion	17/21	-
10-89712015-C-CG	PTEN	c.633_634insG	p.N212Efs*31	SpliceDonorInsertion	6/9	-
10-89712014-G-GC	PTEN	c.633dupC	p.N212Qfs*31	SpliceDonorInsertion	6/9	-
10-89717609-G-GA	PTEN	c.635dupA	p.N212Kfs*31	SpliceAcceptorInsertion	7/9	-
10-89712016-CAGTAAGTG-C	PTEN	c.634_634+7delAGTAAGTG	-	SpliceDonorDeletion	6/9	6/8
17-7577005-TCCTGCTTGCTTA-T	TP53	c.919+2_919+13del12	-	IntronicDeletion	8/11	8/10
17-7576850-T-A	TP53	c.993+3A>T	-	IntronicSNV	-	9/10
1-162741901-G-A	DDR2	c.1592G>A	p.G531E	Missense	13/18	-
15-66774200-A-G	MAP2K1	c.676A>G	p.T226A	Missense	6/11	-
1-162743331-C-A	DDR2	c.1801C>A	p.Q601K	Missense	14/18	-
17-7577549-GCCCATGCAGGAACTGTTA-G	TP53	c.714_731del18	p.N239_G244del	InFrameDeletion	7/11	-
17-37880981-AGCATACGTGATG-A	ERBB2	c.2313_2324del12	p.Y772_A775del	InFrameDeletion	20/27	-
7-55249007-GT-AA	EGFR	c.2305_2306delinsAA	p.V769K	Missense	20/28	-
17-7578257-CC-AT	TP53	c.591_592delinsAT	p.E198*	Missense	6/11	-
1-115256525-TTC-GTCAAG	NRAS	c.184_186delinsCTTGAC	p.E62delinsLD	MultiAAMissense	3/7	-
7-140453158-C-G	BRAF	c.1777G>C	p.G593R	Missense	15/18	-
15-66774119-A-T	MAP2K1	c.595A>T	p.N199Y	Missense	6/11	-
4-55141123-G-C	PDGFRA	c.1769G>C	p.R590T	Missense	12/23	-
2-29443612-C-A	ALK	c.3605G>T	p.G1202V	Missense	23/29	-
17-7577102-CCAGGACAGGCACAAACACGCACCT-C	TP53	c.812_835del24	p.E271_P278del	InFrameDeletion	8/11	-
17-7579359-GGAAACCGTA-T	TP53	c.319_328delinsA	p.Y107_R110delinsS	MultiAAMissense	4/11	-
10-89692781-CC-TT	PTEN	c.265_266delinsTT	p.P89F	Missense	5/9	-
17-7578448-GC-CTGAT	TP53	c.481_483delinsATCAGC	p.A161delinsIS	MultiAAMissense	5/11	-
17-7578201-CAC-AAA	TP53	c.646_648delinsTTT	p.V216F	Missense	6/11	-
10-89720834-A-T	PTEN	c.985A>T	p.N329Y	Missense	8/9	-
17-37879650-C-G	ERBB2	c.2025C>G	p.I675M	Missense	17/27	-
1-243858999-C-T	AKT3	c.66G>A	p.W22*	Nonsense	3/14	-
1-162748464-C-G	DDR2	c.2378C>G	p.S793*	Nonsense	17/18	-
4-55141060-G-A	PDGFRA	c.1706G>A	p.G569E	Missense	12/23	-
3-178927987-T-G	PIK3CA	c.1265T>G	p.L422W	Missense	8/21	-
10-89717709-AGCC-A	PTEN	c.736_738delCCG	p.P246del	InFrameDeletion	7/9	-
19-40762946-G-A	AKT2	c.62C>T	p.T21I	Missense	3/14	-
4-55144122-C-A	PDGFRA	c.1951C>A	p.L651M	Missense	14/23	-
15-66727452-AGAAGCAGAAGGTGGG-A	MAP2K1	c.168_182del15	p.K57_G61del	InFrameDeletion	2/11	-
10-89717704-CCCTCAG-C	PTEN	c.732_737delTCAGCC	p.Q245_P246del	InFrameDeletion	7/9	-
17-7578376-GCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTCACA-G	TP53	c.516_554del39	p.V173_S185del	InFrameDeletion	5/11	-
17-7578446-T-TG	TP53	c.483_483dup	p.I162Hfs*19	Frameshift	5/11	-
17-7578393-CAT-C	TP53	c.536_537delAT	p.H179Rfs*6	Frameshift	5/11	-
17-7578499-CTGCACAGGGCAGGTCTTGG-C	TP53	c.413_431del19	p.A138Gfs*26	Frameshift	5/11	-
10-89720799-T-TA	PTEN	c.951_951dup	p.L318Tfs*7	Frameshift	8/9	-
10-89692985-GA-G	PTEN	c.471delA	p.V158*	Frameshift	5/9	-
17-7577134-TG-T	TP53	c.804delC	p.N268Kfs*77	Frameshift	8/11	-
17-7576912-TGTTGTTGG-T	TP53	c.926_933delCCAACAAC	p.P309Hfs*25	Frameshift	9/11	-
17-7578374-TCGCTATC-T	TP53	c.550_556delGATAGCG	p.D184Mfs*61	Frameshift	5/11	-
17-7578442-GT-G	TP53	c.488delA	p.Y163Sfs*7	Frameshift	5/11	-
7-116414972-GACAAGTGCAGTATCCTC-G	MET	c.3066_3082del17	p.Q1023Dfs*9	Frameshift	15/21	-
17-7578481-G-GTT	TP53	c.448_449insAA	p.T150Kfs*21	Frameshift	5/11	-
17-7578450-C-CA	TP53	c.479_479dup	p.M160Ifs*21	Frameshift	5/11	-
4-55141106-CAAGATGGGA-C	PDGFRA	c.1752_1760delAAGATGGGA	p.R585_E587del	InFrameDeletion	12/23	-
4-55141111-GGGA-G	PDGFRA	c.1759_1761delGAG	p.E587del	InFrameDeletion	12/23	-
17-7578453-GGC-TACAAG	TP53	c.475_477delinsCTTGTA	p.A159delinsLV	MultiAAMissense	5/11	-
17-7578212-CGA-C	TP53	c.636_637delTC	p.F212Lfs*3	Frameshift	6/11	-
17-7577075-ATTCTCTTCCT-A	TP53	c.854_863delAGGAAGAGAA	p.E285Vfs*57	Frameshift	8/11	-
17-7579332-GCTGTCCC-G	TP53	c.349_355delGGGACAG	p.G117Pfs*4	Frameshift	4/11	-
17-7578402-GCAGCGCCT-G	TP53	c.520_527delAGGCGCTG	p.R174Pfs*4	Frameshift	5/11	-