Evolution-guided engineering of small-molecule biosensors.
Nucleic Acids Research
Snoek, Tim T; Chaberski, Evan K EK; Ambri, Francesca F; Kol, Stefan S; Bjørn, Sara P SP; Pang, Bo B; Barajas, Jesus F JF; Welner, Ditte H DH; Jensen, Michael K MK; Keasling, Jay D JD
Facilitating the Evolution of Esterase Activity from a Promiscuous Enzyme (Mhg) with Catalytic Functions of Amide Hydrolysis and Carboxylic Acid Perhydrolysis by Engineering the Substrate Entrance Tunnel.